作者: Heinz Engelhardt , Detlef Mathes
DOI: 10.1016/S0021-9673(01)92047-3
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摘要: Abstract The preparation of chemically bonded stationary phases based on a silica support for aqueous exclusion chromatography is discussed. A defined amount functional groups can be to the surface only if appropriate silane prepared prior binding it surface. properties phases, i.e., wettability by water, adsorption water-soluble polymers, etc., are described. Stationary with amide bonds used separate polystyrenes and polyethylene glycols in dimethylformamide as eluent well dextrans water eluent. graphs molecular weight versus elution volume are, acceptable deviations, independent nature polymer.