Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA.
作者： Fernando Patolsky , Yossi Weizmann , Itamar Willner
DOI: 10.1021/JA0119752
关键词:
摘要: A new concept for the amplified electrochemical detection of 7229-base viral DNA M13φ is developed. thiolated 27-base nucleic acid (1) assembled on an Au-electrode. Hybridization between sensing interface and followed by polymerase-induced replication analyte in presence dCTP, dGTP, dATP, ferrocene-tethered-dUTP (2). The generated redox-active replica mediates electron transfer enzyme glucose oxidase (GOx) electrode activates bioelectrocatalyzed oxidation glucose. provides a biocatalytic amplification path formation replica. techniques to follow bioelectrocatalytic are differential pulse voltammetry cyclic voltammetry. electrical responses from system relate bulk concentration DNA, thus enabling quantitative analysis gene.
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