Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells.

摘要: Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse expressing bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed recombinant vaccinia that can infect promote transient expression full-length mammalian cells. This virus, together another encoding biologically active HIV envelope glycoprotein gp160, allowed us CD4/gp160-mediated cell-cell fusion wide variety nonhuman absence other proteins. By using syncytium formation assays which single type expressed both demonstrated lymphoid nonlymphoid but any 23 tested types, derived from African green monkey, baboon, rabbit, hamster, rat, or mouse. However, mixing experiments one all these could form syncytia when mixed cells; 20 cases, occurred only if molecule was on whereas three be either partners. Interestingly, line, CD4-dependent formed without partner, C-terminally truncated employed. Our results indicate are intrinsically capable undergoing fusion, is usually prevented lack helper presence inhibitory factors membranes.