Immunophenotypic pattern of myeloid populations by flow cytometry analysis.

摘要: We present our experience with immunophenotypic characteristics of benign and malignant myeloid populations, emphasis on differential diagnosis especially between eosinophils, dysplastic granulocytes, neoplastic promyelocytes, monocytes. Eosinophils are characterized by bright CD45, high side scatter (SSC), very low forward (FSC), positive CD11b, CD11c, CD13, CD15, CD33. They negative for CD10, CD14, CD16, CD56, CD64, HLA-DR. Mature monocytes CD33, may express CD2 CD4. Blasts in acute leukemias (AML) minimal differentiation have SSC moderate CD45 expression CD34, CD117, HLA-DR, CD33 be TdT, CD4, CD11c. In promyelocytic leukemia (APL), four FC patterns can recognized. The majority cases represented classical (hypergranular) APL were SSC, usually heterogeneous (pattern 1). second most common type, corresponding to hypogranular (microgranular) variant APL, differed from frequent coexpression CD34 2). Rare (pattern3) showed mixture cells (SSC(low)/CD2(+)/CD13(+)/CD33(+)/CD34(+)/CD117(+)) prominent population granulocytes/maturing precursors (SSC(high)/CD10(+/-)/CD16(+/()/CD117(()). One case two one hypergranular 4). Detailed phenotypic granulocytes their also presented.