Hormonal antagonistic properties of chemically deglycosylated human choriogonadotropin.
作者： M R Sairam , P Manjunath
DOI: 10.1016/S0021-9258(18)33276-9
关键词:
摘要: The biological properties of chemically deglycosylated human choriogonadotropin (DG-hCG) preparations were examined in collagenase-dispersed rat interstitial cells vitro. Despite effective receptor binding activity membrane preparations, DG-hCG failed to induce cyclic AMP accumulation the when incubated presence or absence a phosphodiesterase inhibitor. steroidogenic ability as assessed by testosterone medium was less than 0.5% native hormone with failure attain maximal steroid production. Time course experiments have revealed that altered kinetics could not be responsible for loss response. Consistent its property good and poor cell activation, antagonized action hCG. When added at same time, inhibited stimulatory dose hCG dose-dependent manner. Inhibition complete whereas inhibition steroidogenesis about 75%. had no effect on cholera toxin follitropin seminiferous tubular preparations. data suggest has conformation conductive interaction receptor, but activate adenylate cyclase is either lost weakly expressed.
sci-hub.st 参考文章(16)
P Manjunath, M R Sairam, Biochemical, biological, and immunological properties of chemically deglycosylated human choriogonadotropin. Journal of Biological Chemistry. ,vol. 257, pp. 7109- 7115 ,(1982) , 10.1016/S0021-9258(18)34544-7
M L Dufau, K Hayashi, K J Catt, P M Conn, T Tsuruhara, K A Horner, Actions of choleragen and gonadotropin in isolated Leydig cells. Functional compartmentalization of the hormone-activated cyclic AMP response. Journal of Biological Chemistry. ,vol. 253, pp. 3721- 3729 ,(1978) , 10.1016/S0021-9258(17)34861-5
M.R. Sairam, Studies on pituitary follitropin Archives of Biochemistry and Biophysics. ,vol. 194, pp. 63- 70 ,(1979) , 10.1016/0003-9861(79)90595-2
W. R. MOYLE, J. RAMACHANDRAN, Effect of LH on steroidogenesis and cyclic AMP accumulation in rat Leydig cell preparations and mouse tumor Leydig cells. Endocrinology. ,vol. 93, pp. 127- 134 ,(1973) , 10.1210/ENDO-93-1-127
Cornelia P. Channing, Om P. Bahl, Role of Carbohydrate Residues of Human Chorionic Gonadotropin in Stimulation of Progesterone Secretion by Cultures of Monkey Granulosa Cells Biology of Reproduction. ,vol. 18, pp. 707- 711 ,(1978) , 10.1095/BIOLREPROD18.5.707
A. Jagannadha Rao, J. Ramachandran, Cyclic AMP production in isolated rat seminiferous tubule cell preparations: a potential in vitro assay for follicle stimulating hormone. Life Sciences. ,vol. 17, pp. 411- 416 ,(1975) , 10.1016/0024-3205(75)90491-9
M. R. SAIRAM, M. CHRÉTIEN, CHOH HAO LI, On the isolation of human pituitary hormones. The Journal of Clinical Endocrinology and Metabolism. ,vol. 47, pp. 1002- 1008 ,(1978) , 10.1210/JCEM-47-5-1002
A. G. Gilman, A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate Proceedings of the National Academy of Sciences of the United States of America. ,vol. 67, pp. 305- 312 ,(1970) , 10.1073/PNAS.67.1.305
Kanji Sato, Yukitaka Miyachi, Nakaaki Ohsawa, Kinori Kosaka, In vitro stimulation of steroidogenesis in rat testis by cholera enterotoxin. Biochemical and Biophysical Research Communications. ,vol. 62, pp. 696- 703 ,(1975) , 10.1016/0006-291X(75)90455-6
P. Manjunath, M.R. Sairam, J. Sairam, Studies on pituitary follitropin X. Biochemical, receptor binding and immunological properties of deglycosylated ovine hormone Molecular and Cellular Endocrinology. ,vol. 28, pp. 125- 138 ,(1982) , 10.1016/0303-7207(82)90026-0