The genes involved in asthma with the treatment of human embryonic stem cell-derived mesenchymal stem cells

摘要: Abstract Background Asthma is affecting more than 300 million people worldwide, which represents the most common chronic disease among children. We previously found that mesenchymal stem cells (MSCs) derived from induced pluripotent (iPSCs) modulated immune response on Th2-mediated asthma in vivo and vitro. This study further evaluated immunomodulatory effects of MSCs human embryonic (hESCs) asthma. Methods Multipotent hESC-MSCs were obtained using a feeder-free method. The analysed for expression cell surface markers by flow cytometry, their differentiation potentials vitro trilineage methods transplanted into murine model with ovalbumin (OVA)-induced airway allergic inflammation. levels related genes measured mRNA PCR arrays. Results expressed classical MSC held capability multiple mesoderm-type lineages. able to suppress inflammation modulating Th2 eosinophils mice, reversed reduction regulatory T cells. By array, 5 mRNAs- chemokine (C-C motif) ligand 11 (Ccl11), Ccl24, interleukin13 (Il13), Il33 eosinophil-associated, ribonuclease A family, member (Ear11) identified relevant treatment. Conclusions therapeutic key mRNAs involved. will provide better understanding regarding mechanisms underlying application