Catabolism of [4-14C]testosterone by subcellular fractions of human prostate.

摘要: 1. Incubation conditions were established in experiments with human-prostate homogenates for almost complete conversion of [4-(14)C]testosterone into at least ten transformation products. 2. Whole tissue benign hypertrophy shown to contain 3alpha-, 3beta- and 17beta-hydroxy steroid dehydrogenases, Delta(4)-3-oxo 5alpha- 5beta- reductases unidentified hydroxylases. 3. Most the steroid-dehydrogenase activity was located mitochondria, which showed little other activity. 4. The 3alpha- 3beta-hydroxy dehydrogenases 5beta-reductase high-speed supernatant required supplementation NADPH 5. 5alpha-reductase both microsomal fractions also NADPH.