Identification of kelp zoospores from in situ plankton samples

摘要: Studies of the link between planktonic and benthic stages marine macroalgae have been hampered by inability to identify from field samples. Here I describe a microphotometric method for identifying kelp zoospores in situ plankton samples based on light-absorbance characteristics zoospore plastids. Absorption spectra were obtained plastids within Macrocystis pyrifera (L.) C. Agardh, Pterygophora californica Ruprecht, Eisenia arborea Areschoug Laminaria farlowii Setchell released laboratory adult specimens collected field; these are dominant subtidal kelps most southern California forests. highly specific each species. Average absorbance decreased P. E. L. M. at wavelengths 400 750 nm. Discriminant-function analyses provided significant discriminant rule, zoospore-plastid values 25 different wavelengths, unknown among four Cross-validation techniques indicated that rule could successfully 97.8% pyrifera, 96.4% californica, 86.2% 69.7% zoospores. Most misidentified identified as farlowii, vice-versa; when relatively rare was pooled with treated single complex, 98.2% 97.4% 89.5% arborea/L. Coupled protocol isolating non-kelp cells, this used determine abundances species-compositions This is first description macroalgal or spores