Genome-wide DNA methylation analysis of human brain tissue from schizophrenia patients

摘要: Recent studies suggest that genetic and environmental factors do not account for all the schizophrenia risk, epigenetics also has a role in disease susceptibility. DNA methylation is heritable epigenetic modification can regulate gene expression. Genome-wide analysis was performed on post-mortem human brain tissue from 24 patients with unaffected controls. assessed at over 485,000 CpG sites using Illumina Infinium HumanMethylation450 Bead Chip. After adjusting age interval, 4641 probes corresponding to 2929 unique genes were found be differentially methylated. Of those genes, 1291 located island 817 promoter region. These include NOS1, AKT1, DTNBP1, DNMT1, PPP3CC SOX10, which have previously been associated schizophrenia. More than 100 of these overlap previous study peripheral blood 27,000 analysed. Unsupervised clustering top 3000 most variable revealed two distinct groups significantly more people cluster one compared controls (P=1.74 × 10(-4)). The first composed 88% only 12% controls, whereas second 27% 73% results strongly differential important etiology add support use profiles as future prognostic indicator