Dexamethasone modulates osteogenesis and adipogenesis with regulation of osterix expression in rat calvaria-derived cells

摘要: Osteoblasts and adipocytes originate from common mesenchymal progenitor cells although a number of compounds can induce osteoblastic adipogenic differentiation cells, the underlying mechanisms have not been elucidated. The present study examined synergistic effects dexamethasone (Dex) bone morphogenetic protein (BMP)-2 on clonal isolated rat calvaria into osteoblasts adipocytes, as well timing treatment. Cells were cultured for various periods time in presence Dex and/or BMP-2. When treated with Dex+BMP-2 during early phase differentiation, they differentiated adipocytes. However, when late effect vitro matrix mineralization was observed. To examine differences between phases ALP activity measured increased markedly Day 9, corresponding to onset Dex. treatment inhibited osterix (OSX) expression committed but osteogenic following BMP-2 isoform2 OSX promoter region found be involved differentiation. Furthermore, stably expressing (isoform2) formed mineralized nodules even though had It appears that modulates osteogenesis adipogenesis stem by regulating expression.