Effects of ciprofibrate on testicular and adrenal steroidogenic enzymes in the rat
作者： Andrée M. Hierlihy , Gerard M. Cooke , Ivan H.A. Curran , Rekha Mehta , Litsa Karamanos
DOI: 10.1016/J.REPROTOX.2005.11.001
关键词:
摘要: Testicular and adrenal steroidogenic enzymes were measured radiometrically following oral dosing of rats with ciprofibrate (2-[4-(2,2-dichlorocyclopropyl) phenoxyl]-2-methylpropinoic acid), a peroxisome proliferator. Six-week-old male Fisher 344 fed diet containing (0.025%, w/w) for 3, 7, 14, 28, 56, 84, 112 or 140 days leading to daily intake approximately 15 mg/kg body weight/day. Ciprofibrate caused marked inhibition testicular 3β-hydroxysteroid dehydrogenase-isomerase (3β-HSD) activity that was significant after 3 subsequently decreased 40% control level. treatment also reduced 17β-hydroxysteroid dehydrogenase (17β-HSD) lesser extent but had no effect on 17-hydroxylase (17-OHase) activity. Immunoblot analyses indicated did not alter enzyme protein levels semi-quantitative RT-PCR analysis revealed changes in 3β-HSD mRNA levels. Furthermore, addition the enzyme-specific testes, tissue-specific evident, since effects seen activities 21-OHase glands from same animals.
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