Precision is essential for efficient catalysis in an evolved Kemp eliminase
作者： Rebecca Blomberg , Hajo Kries , Daniel M. Pinkas , Peer R. E. Mittl , Markus G. Grütter
DOI: 10.1038/NATURE12623
关键词:
摘要: … Starting from a computationally designed catalyst for the Kemp elimination 3 —a well-studied model system for proton transfer from carbon—we show that an artificial enzyme can be …
rcsb.org
core.ac.uk
harvard.edu
nature.com参考文章(47)
DV Goeddel, E Chen, DW Leung, A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction Technique. ,vol. 1, pp. 11- 15 ,(1989)
Sylvia J. Putman, A. F. W. Coulson, I. R. T. Farley, B. Riddleston, J. R. Knowles, Specificity and kinetics of triose phosphate isomerase from chicken muscle Biochemical Journal. ,vol. 129, pp. 301- 310 ,(1972) , 10.1042/BJ1290301
W. John Albery, Jeremy R. Knowles, Evolution of enzyme function and the development of catalytic efficiency Biochemistry. ,vol. 15, pp. 5631- 5640 ,(1976) , 10.1021/BI00670A032
Jeremy R. Knowles, Enzyme catalysis: not different, just better. Nature. ,vol. 350, pp. 121- 124 ,(1991) , 10.1038/350121A0
Richard David Gandour, On the importance of orientation in general base catalysis by carboxylate Bioorganic Chemistry. ,vol. 10, pp. 169- 176 ,(1981) , 10.1016/0045-2068(81)90020-1
Christopher A. Voigt, D.Benjamin Gordon, Stephen L. Mayo, Trading accuracy for speed: A quantitative comparison of search algorithms in protein sequence design. Journal of Molecular Biology. ,vol. 299, pp. 789- 803 ,(2000) , 10.1006/JMBI.2000.3758
Yunfeng Hu, K. N. Houk, Kazuya Kikuchi, Kinya Hotta, Donald Hilvert, Nonspecific Medium Effects versus Specific Group Positioning in the Antibody and Albumin Catalysis of the Base-Promoted Ring-Opening Reactions of Benzisoxazoles Journal of the American Chemical Society. ,vol. 126, pp. 8197- 8205 ,(2004) , 10.1021/JA0490727
Stephen C. Blacklow, Ronald T. Raines, Wendell A. Lim, Philip D. Zamore, Jeremy R. Knowles, Triosephosphate isomerase catalysis is diffusion controlled. Appendix: Analysis of triose phosphate equilibria in aqueous solution by 31P NMR. Biochemistry. ,vol. 27, pp. 1158- 1167 ,(1988) , 10.1021/BI00404A013
John P. Richard, Acid-base catalysis of the elimination and isomerization reactions of triose phosphates Journal of the American Chemical Society. ,vol. 106, pp. 4926- 4936 ,(1984) , 10.1021/JA00329A050
K KIKUCHI, R HANNAK, M GUO, A KIRBY, D HILVERT, Toward bifunctional antibody catalysis. Bioorganic & Medicinal Chemistry. ,vol. 14, pp. 6189- 6196 ,(2006) , 10.1016/J.BMC.2006.05.071