Silver nanoparticles (AgNPs) and AgNO3 perturb the specification of human hepatocyte-like cells and cardiomyocytes.

摘要: Silver nanoparticles (AgNPs) are commonly utilized industrial compounds mostly because of their antimicrobial properties. Nevertheless, our understanding potential developmental toxicity in humans is still limited. Embryonic stem cells (ESCs) powerful vitro tools for assessments chemicals. Here, we evaluated the during early embryogenesis AgNPs and AgNO3 with human ESC (hESC)-based differentiation systems vitro. We found that relevant concentrations Ag ions affected specification two three primary germ layers, endoderm mesoderm, without drastically affecting ectoderm. Furthermore, forms impaired generation functions hepatocytes-like derived from endoderm, by decreasing expression important liver markers such as AFP, ALB, HNF4A, altering glycogen storage. When considering cardiac development, manifested opposite adverse effects, increased while decreased typical (NKX2.5, MYH6, ISL) hESC-derived cardiomyocytes. In conclusion, findings argue a AgNP doses exposed to, or levels detected body, especially at very stages embryogenesis, which may not be just due to leakage. Moreover, mesendoderm-derived cell types, tissues organs more prone than ectoderm lineages.