The salmonid myostatin gene family: a novel model for investigating mechanisms that influence duplicate gene fate

摘要: Most fishes possess two paralogs for myostatin, a muscle growth inhibitor, while salmonids are presumed to have four: mstn1a, mstn1b, mstn2a and mstn2b, pseudogene. The mechanisms responsible preserving these duplicates as well the depth of mstn2b nonfunctionalization within family remain unknown. We therefore characterized several genomic clones in order better define species gene phylogenies. Gene organization sequence conservation was particularly evident among paralog groupings salmonid subfamilies. All sequences included in-frame stop codons, confirming its across taxa, although indels polymorphisms often differed. For example, specific Onchorhynchus tshawytscha O. nerka genes were remarkably similar differed equally from other orthologs. A phylogenetic analysis weakly established clade including only species, which coupled with shared 51 base pair deletion might suggest history involving hybridization or history. Furthermore, mstn2 introns all lacked conserved splice site motifs, suggesting that tissue-specific processing transcripts, but not those is due alternative cis regulation likely common feature salmonids. It also suggests limited transcript may contributed nonfunctionalization. Previous studies revealed divergence promoters current provide evidence relaxed positive selection some coding lineages. These results together myostatin novel resource investigating regulate duplicate fate differences expression, protein structure suggestive active divergence.