Separation of the Spores and Crystals of Bacillus thuringiensis

摘要: THE isolation of the protein crystals Bacillus thuringiensis, which are toxic to larvae certain species insects, has been described previously1,2, and involves phase separation in an aqueous consisting a suspension spores is emulsified with variable volume organic solvent such as trifluorotrichloroethane. When two phases mixed, most some removed from into left phase. An ideal method would recover 100 per cent completely free spores. This not yet achieved; usually greater degree crystal purity required, lower yield crystals.