Differential expression of DNA repair machinery genes in normal bronchial tissue and non-small cell lung cancer (NSCLC)

摘要: 22017 Background: Genes involved in DNA repair and/or replication have been recently investigated as predictive markers of response to chemotherapy NSCLC. However, few data on the expression these genes NSCLC tumor samples versus corresponding normal lung are currently available. Aim this study was evaluate differential mRNA levels 22 belonging 5 different pathways: direct (DR), base excision (BER), nucleotide (NER), double strand break (DSBR) and post-replicative (PRR) repair. In addition, 6 (REP) 2 telomere maintenance (TM) were also investigated. Methods: Total RNA extracted from fresh snap-frozen tumors tissues 50 consecutive chemo-naive resected patients. Transcript quantified by Real-Time PCR (TaqMan), fold changes calculated with 2-ΔΔCt method statistical significance assessed Wilcoxon's test. POLR2A 18SrRNA used refere...