Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus.

摘要: Alfalfa mosaic virus (AMV) is a worldwide distributed that has very wide host range and causes significant crop losses of many economically important crops, including potato (Solanum tuberosum L.). In this study, the antiviral activity Bacillus licheniformis strain POT1 against AMV on plants was evaluated. The dual foliar application culture filtrate (CF), 24 h before after AMV-inoculation, most effective treatment showed 86.79% reduction viral accumulation level improvement different growth parameters. Moreover, HPLC analysis 20 polyphenolic compound accumulated with total amount 7,218.86 1606.49 mg/kg in POT1-treated non-treated plants, respectively. Additionally, transcriptional thirteen genes controlling phenylpropanoid, chlorogenic acid flavonoid biosynthetic pathways revealed studied were induced treatments. stronger expression F3H, key enzyme biosynthesis (588.133-fold) AN2, anthocyanin 2 transcription factor, (97.005-fold) suggested flavonoid, especially anthocyanin, might play roles plant defense infection. Gas chromatography-mass spectrometry (GC-MS) pyrrolo[1,2-a]pyrazine-1,4-dione major CF ethyl acetate extract, suggesting it acts as elicitor molecules for induction systemic acquired resistance plants. To our knowledge, first study biological control mediated by PGPR