Isolation of Y chromosome-specific sequences from Silene latifolia and mapping of male sex-determining genes using representational difference analysis.

摘要: The genomic subtraction method representational difference analysis (RDA) was used to identify male-specific restriction fragments in the dioecious plant Silene latifolia. Male-specific are linked male sex chromosome (the Y chromosome). Four RDA-derived were polymorphisms a collection of X-ray-generated mutant plants with either hermaphroditic or asexual flowers. Some mutants have cytologically detectable deletions that correlated loss fragments. One probe detected fragment present all mutants, indicating each has retained chromosomal DNA. other three probes region deleted some and mutants. Based on phenotypes correlation visible fragments, these markers assigned positions close carpel suppression locus. This RDA mapping also revealed Y-linked locus, not previously described, which is responsible for early stamen development.