The cilia protein IFT88 is required for spindle orientation in mitosis

摘要: Cilia dysfunction has long been associated with cyst formation and ciliopathies. More recently, misoriented cell division observed in cystic kidneys, but the molecular mechanism leading to this abnormality remains unclear. Proteins of intraflagellar transport (IFT) machinery are linked cystogenesis required for cilia non-cycling cells. Several IFT proteins also localize spindle poles mitosis, indicating uncharacterized functions these dividing Here, we show that IFT88 depletion induces mitotic defects human cultured cells, kidney cells from mouse mutant Tg737(orpk) zebrafish embryos. In is part a dynein1-driven complex transports peripheral microtubule clusters containing microtubule-nucleating ensure proper astral arrays thus orientation. This work identifies protein orientation important implications etiology