Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

作者: NI KADEK DWIPAYANI LESTARI , NI WAYAN , DESWINIYANTI , IDA AYU ASTARINI , LUH MADE ARPIWI

DOI: 10.13057/NUSBIOSCI/N110209

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摘要: Abstract. Lestari NKD, Deswiniyanti NW, Astarini IA, Arpiwi NLM. 2019. Callus and shoot induction of leaf culture Lilium longiflorum with NAA BAP. Nusantara Bioscience 11: 162-165. Thunb., an Easter lily, is a common ornamental plant used as potted plant, cut flower, cosmetic medicine. The research on tissue technique to induce shoots callus expected increase the yield lilies quickly efficiently. This study aims determine effect growth regulator combination Naphthalene Acetic Acid (NAA) Benzyl Amino Purine (BAP) development best concentration for lily plants. was conducted in completely randomized design using explants Vacin Went medium, BAP (0; 0.5; 1 mg..L-1 ) treatments. result eight-week observations shows that regulators significantly affected either days initiation callus, shoot, percentage diameter no length shoot. mg.L-1 had forming (100%), means number (5.8), (11.6 cm).

参考文章(17)
Wiry Pungkastiani, Dwi Hapsoro, Yusnita, IN VITRO ORGANOGENESIS OF TWO SANSEVIERIA CULTIVARS ON DIFFERENT CONCENTRATIONS OF BENZYLADENINE (BA) Agrivita : Journal of Agricultural Science. ,(2011)
Jitender Kumar Kanwar, Pawan Kumar Mahajan, Ranjana Kapoor, Surinder Kumar, In vitro bulblet productivity in different explants of hybrid lilies Journal of fruit and ornamental plant research. ,vol. 16, pp. 345- 352 ,(2008)
A. K. Singh, R. K. Pandey, Mamta Sharma, In vitro propagation of Lilium. Biological Forum. ,vol. 1, pp. 20- 22 ,(2009)
Chang-Tesrn Chen, Chen Chang, Yu-Ching Tsai, Wei-Chin Chang, A Tissue Culture Protocol for Propagation of a Rare Plant, Lilium speciosum Thunb. var. gloriosoides Baker Botanical Bulletin of Academia Sinica. ,vol. 41, pp. 139- 142 ,(2000) , 10.7016/BBAS.200004.0139
Xu LingFei, Ma FengWang, Liang Dong, Plant regeneration from in vitro cultured leaves of Lanzhou lily (Lilium davidii var. unicolor) Scientia Horticulturae. ,vol. 119, pp. 458- 461 ,(2009) , 10.1016/J.SCIENTA.2008.08.026
Yoshiji Niimi, In Vitro Propagation and Post-in Vitro Establishment of Bulblets of Lilium japonicum Thunb. Journal of the Japanese Society for Horticultural Science. ,vol. 63, pp. 843- 852 ,(1995) , 10.2503/JJSHS.63.843
A.H. Naing, H. Yun, J. Lucidos, Y.J. Hwang, C.K. Kim, B.J. Ahn, K.B. Lim, Plant regeneration through various explants of Lilium longiflorum hybrid “Bright Tower” and determination of ploidy level of regenerated plants Plant Biosystems. ,vol. 148, pp. 55- 56 ,(2014) , 10.1080/11263504.2012.754384
Didier Reinhardt, Therese Mandel, Cris Kuhlemeier, Auxin Regulates the Initiation and Radial Position of Plant Lateral Organs The Plant Cell. ,vol. 12, pp. 507- 518 ,(2000) , 10.1105/TPC.12.4.507