Enzyme-linked immunosorbent assay for chlamydial antibodies.

作者: V J Lewis , W L Thacker , S H Mitchell

DOI: 10.1128/JCM.6.5.507-510.1977

关键词:

摘要: An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The antigen produced cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for by a complement-fixation test were at even higher dilutions ELISA. Paired diagnostic rises complement-fixing antibody showed seroconversion ELISA also. Control from persons no history of infection negative both tests. Sera patients psittacosis or lymphogranuloma venereum positive, indicating that the used this study is genus specific rather than species specific.

参考文章(19)
A. Voller, D. E. Bidwell, A simple method for detecting antibodies to rubella. British journal of experimental pathology. ,vol. 56, pp. 338- 339 ,(1975)
Bidwell D, Engvall E, Huldt G, Voller A, A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bulletin of The World Health Organization. ,vol. 51, pp. 209- 211 ,(1974)
D. E. Bidwell, A. Voller, Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. British journal of experimental pathology. ,vol. 57, pp. 243- 247 ,(1976)
Ernest Jawetz, William D. Linscott, Julius Schachter, S. Vern Juchau, Inhibition of Antichlamydial IgM Antibody by IgG Antibody in Immunofluorescence Tests Journal of Immunology. ,vol. 108, pp. 1563- 1569 ,(1972)
B. J. M. Brosi, Janny Buys, P. A. Steerenberg, E. J. Ruitenberg, Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays Bulletin of The World Health Organization. ,vol. 51, pp. 108- 109 ,(1974)
Vester J. Lewis, W. Lanier Thacker, Helen M. Engelman, Indirect Hemagglutination Test for Chlamydial Antibodies Applied Microbiology. ,vol. 24, pp. 22- 25 ,(1972) , 10.1128/AM.24.1.22-25.1972
H Russell, L R Edwards, R R Facklam, Enzyme-linked immunosorbent assay for streptococcal M protein antibodies. Journal of Clinical Microbiology. ,vol. 3, pp. 501- 505 ,(1976) , 10.1128/JCM.3.5.501-505.1976
Lavelle Hanna, Ernest Jawetz, Isao Hoshiwara, Chandler Dawson, Bechir Nabli, Bruce Ostler, Titration and Typing of Serum Antibodies in Tric Infections by Immunofluorescence Journal of Immunology. ,vol. 108, pp. 102- 107 ,(1972)
Nicholas Hahon, Kenneth O. Cooke, Fluorescent Cell-Counting Neutralization Test for Psittacosis Journal of Bacteriology. ,vol. 89, pp. 1465- 1471 ,(1965) , 10.1128/JB.89.6.1465-1471.1965