Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia Pseudomallei Flagellin.

摘要: Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, serious and often fatal infectious disease that poorly controlled by existing treatments. Due to its inherent resistance the major antibiotic classes facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention therapeutic management. One of main subunit candidates flagellin (FliCBp). Here, we present high resolution crystal structure FliCBp report synthesis characterization three peptides predicted both B T cell epitopes, structure-based in silico methods, sequence-based epitope prediction tools. All epitopes were shown immunoreactive against human IgG antibodies elicit cytokine production from peripheral blood mononuclear cells. Furthermore, two (F51-69 F270-288) found dominant their enhanced bactericidal activities purified neutrophils. The derived this study may represent potential melioidosis components.