A previously undescribed coronavirus associated with respiratory disease in humans.

摘要: The etiology of acute respiratory tract illnesses is sometimes unclear due to limitations diagnostic tests or the existence as-yet-unidentified pathogens. Here we describe identification and characterization a not previously recognized coronavirus obtained from an 8-mo-old boy suffering pneumonia. This replicated efficiently in tertiary monkey kidney cells Vero cells, contrast human coronaviruses (HCoV) 229E OC43. entire cDNA genome sequence undescribed was determined, revealing that it most closely related porcine epidemic diarrhea virus HCoV 229E. maximum amino acid identity between ORFs newly discovered group 1 ranged 43% 67%. Real-time RT-PCR assays were designed test for prevalence humans. Using these tests, detected four 139 individuals (3%) who illness with unknown etiology. All patients suffered fever, runny nose, dry cough, all had underlying additional morbidity. Our data will enable development study clinical impact this humans more detail. Moreover, be important discriminate OC43 severe syndrome coronavirus.