High-Resolution 2D NMR Spectroscopy of Bicelles To Measure the Membrane Interaction of Ligands

摘要: Magnetically aligned bicelles are increasingly being used as model membranes in solution- and solid-state NMR studies of the structure, dynamics, topology, interaction membrane-associated peptides proteins. These commonly utilize PISEMA pulse sequence to measure dipolar coupling chemical shift, two key parameters subsequent structural analysis. In present study, we demonstrate that other rotating-frame sequences not suitable for measurement long-range heteronuclear couplings, they provide inaccurate values when multiple protons coupled a 13C nucleus. Furthermore, laboratory-frame separated-local-field experiment is capable overcoming these difficulties magnetically bicelles. An extension this approach accurately 13C−31P 1H−31P couplings from phospholipids, which useful understand molecules with membrane, also described. In...