Methods for producing hyaluronan in a recombinant host cell
作者： William Widner , Maria Tang , Regine Behr , Alan Sloma , Stephen Brown
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摘要: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating Bacillus host cell under conditions suitable production of the wherein comprises nucleic acid construct comprising hyaluronan synthase encoding sequence operably linked promoter foreign sequence; and (b) recovering from cultivation medium. also an isolated operon gene UDP-glucose 6-dehydrogenase gene, optionally one or more genes selected group consisting pyrophosphorylase UDP-N-acetylglucosamine glucose-6-phosphate isomerase gene. sequences 6-dehydrogenase, pyrophosphorylase, pyrophosphorylase.
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