Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.

摘要: Abstract Unique-sequence synthetic DNA probes, based on the known amino acid sequence of bovine pancreatic trypsin inhibitor, were constructed from oligodeoxynucleotides. In genomic Southern blot experiments, these probes shown to hybridize specifically discrete restriction fragments. A probe also was used isolate a cloned BPTI gene library. analysis this clone indicated that coding region neither preceded by start codon nor immediately followed termination codon. This suggests mature form may be produced through proteolytic processing larger polypeptide precursor.