The over-expression of aquaporin-1 alters erythroid gene expression in human erythroleukemia K562 cells

摘要: Aquaporin genes are differentially expressed in primitive versus definitive erythropoiesis. Our previous research results showed that over-expression of aquaporin-1 (AQP1) gene greatly promotes the erythroid differentiation erythroleukemia K562 cells, using benzidine staining and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis for representative erythroid-related genes, including γ-globin. But molecular mechanisms underlying erythroid-specific regulation remain unknown. In this study, we demonstrated AQP1 induced hemoglobins expression altered by microarray cells. The retroviral vector (pBABE-puro-AQP1) was constructed infected cells to establish a stable cell line (K562-AQP1). effectively inhibited proliferation growth arrest G1 phase Then profile applied analyze which involved mechanism induction. DAVID functional annotation clustering tool used identify biological functions enriched with (n = 466 genes) group into clusters based on their similarity. Significant enrichment “oxygen transporter activity” (p = 3.8E-7) (HBD, HBG, HBB, HBE1, HBQ1), HEMGN, EBP42 were validated qRT-PCR. Moreover, silencing HEMGN RNA interference K562-AQP1 resulted down-regulation these genes. These data provide better understanding role differentiation, promoting induction other potential signaling pathways associated hemoglobin