Selective and sensitive fluorescence "turn-on" detection of 4-thiouridine in nucleic acids via oxidative amination.
作者： Jingyi Wang , Jiachen Shang , Zichen Qin , Aijun Tong , Yu Xiang
DOI: 10.1039/C9CC06312A
关键词:
摘要: A fluorescence "turn-on" method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed this work based on the oxidative amination s4U by fluoresceinamine (FAM-NH2) and periodate (IO4-). It 125-fold more sensitive detection than traditional UV330 absorption method, showed excellent selectivity to over 2-thiouridine (s2U) analogues biological thiols.
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