Genomic Characterization of the Novel Aeromonas hydrophila Phage Ahp1 Suggests the Derivation of a New Subgroup from phiKMV-Like Family.

摘要: Aeromonas hydrophila is an opportunistic pathogenic bacterium causing diseases in human and fish. The emergence of multidrug-resistant A. isolates has been increasing recent years. In this study, we have isolated a novel virulent podophage hydrophila, designated as Ahp1, from waste water. Ahp1 rapid adsorption (96% adsorbed 2 min), latent period 15 min, burst size 112 PFU per infected cell. At least eighteen virion proteins were visualized SDS-polyacrylamide gel electrophoresis, with 36-kDa protein being the predicted major capsid protein. Genome analysis revealed linear doubled-stranded DNA genome 42,167 bp G + C content 58.8%. encodes 46 putative open reading frames, 5 phage promoters, 3 transcriptional terminators. Based on high degrees similarity overall organization among most corresponding ORFs, well phylogenetic relatedness their DNAP, RNAP proteins, propose new subgroup, Ahp1-like subgroup. This subgroup contains members previously belonging to phiKMV-like phiAS7, phi80-18, GAP227, phiR8-01, ISAO8. Since narrow host range, for effective therapy, different phages are needed preparation cocktails that capable killing heterogeneous strains.