作者: M. So , B. J. McCarthy
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摘要: Abstract the Escherichia coli heat-stable toxin (ST I) is encoded within a transposon (Tn1681) flanked by inverted repeats of insertion sequence 1 (IS1) [So, M., Heffron, F. & McCarthy, B. J. (1979) Nature (London) 277, 453-456]. By subcloning restriction fragments and mutagenesis, we located precisely the gene for ST I transposon. We determined complete nucleotide central portion Tn1681 (i.e., that part IS1) identified coding toxin. From sequence, deduced probable amino acid I. The NH2-terminal extremely hydrophobic bears striking resemblance to signal fd phage minor coat protein. using subcloned fragment containing but no IS1 sequences, (i) with activity assayable in suckling mice genetically distinct from St ligated ileal loops II) (ii) can be responsible diarrheal disease different animals.