Effect of Praziquantel on Schistosoma mekongi Proteome and Phosphoproteome.

摘要: Schistosoma mekongi causes schistosomiasis in southeast Asia, against which praziquantel (PZQ) is the only treatment option. PZQ resistance has been reported, thus increasing requirement to understand mechanism of PZQ. Herein, this study aimed assess differences proteome and phosphoproteome S. after for elucidating its action. Furthermore, key kinases related effects were predicted identify alternative targets novel drug development. Proteomes profiled at half maximal inhibitory concentration compared with untreated worms. A total 144 proteins differentially expressed treatment. In parallel, immunohistochemistry indicated a reduction phosphorylation, 43 phosphoproteins showing reduced as identified by phosphoproteomic approach. Pathway analysis mass spectrometric data showed that calcium homeostasis, worm antigen, oxidative stress pathways influenced Interestingly, two mechanisms protein folding proteolysis through endoplasmic reticulum-associated degradation parasiticidal According kinase-substrate predictions bioinformatic tools, Src kinase was highlighted major alteration phosphorylation Interfering these or applying inhibitors could be approaches further antischistosomal