Counting low-copy number proteins in a single cell.
作者： B. Huang , H. Wu , D. Bhaya , A. Grossman , S. Granier
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摘要: We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of single cell using single-molecule fluorescence counting. Generic labeling proteins is achieved through fluorescent-antibody binding. The use cylindrical optics enables high-efficiency (≈60%) counting molecules micrometer-sized channels. used this to β 2 adrenergic receptors expressed insect cells (SF9). also analyzed phycobiliprotein individual cyanobacterial ( Synechococcus sp. PCC 7942) observed marked differences levels specific complexes populations that were grown under nitrogen-depleted conditions.
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