In vitro investigation of anticancer, cell-cycle-inhibitory, and apoptosis-inducing effects of diversin, a natural prenylated coumarin, on bladder carcinoma cells.

摘要: Chemotherapy is one of the main strategies for reducing rate cancer progression or, in some cases, curing tumour. Since a great number chemotherapeutic agents are cytotoxic compounds, i. e. similarly affect normal and neoplastic cells, application antitumour drugs preferred management therapy. In this study, cytotoxicity diversin was evaluated 5637 transitional cell carcinoma (TCC) subline (bladder carcinoma), human fibroblast cells using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Chromatin condensation DNA damage induced by were also determined means 4',6-diamidino-2-phenylindole (DAPI) staining comet assay, respectively. addition, mechanism action studied more detail caspase 3 colourimetric assay flow cytometry-based cell-cycle analyses (PI staining). Our results revealed that has considerable effects but not on HFF3 (human foreskin fibroblast) HDF1 dermal cells. Further studies showed exerts its via induction chromatin condensation, damage, activation cytometric mostly arrested at G2 phase cycle presence diversin.