A new technique for modeling neuronal connectivity using human pluripotent stem cells

摘要: Purpose: We describe a technique for independently differentiating neocortical and mesencephalic dopaminergic (mDA) neurons from single human pluripotent stem cell (hPSC) line, subsequently allowing the two types to interact form connections. Methods: Dopaminergic progenitors were differentiated in separate vessels, then separately seeded into inner outer compartments of specialized culture vessels designed vitro studies wound healing. Cells further using dopamine-specific neocortex-specific trophic factors, respectively. The barrier was removed, differentiation continued three weeks presence BDNF. Results: After differentiation, mDA bodies largely remained areas which they had been seeded, gap between neuron populations could still be discerned. Abundant tyrosine hydroxylase (TH)-positive projections extended area chamber area. Conclusions: have developed hPSC-based system producing connections brain regions, neocortex midbrain. Future experiments employ modifications this method examine any regions or neuronal subtypes that can produced hPSCs vitro.