Validation of flow cytometry for rapid enumeration of bacterial concentrations in pure cultures

摘要: Flow cytometry was investigated as a rapid detection and counting method for bacteria in pure cultures. A simple two-parameter scheme employed: particle size measured by forward angle light scatter nucleic acid content fluorescence of the DNA/RNA-binding dye ethidium bromide. The technique gave results that correlated exceptionally well with conventional plate four species bacteria, concentrations range 10(2) to 10(7) cfu/ml. Cytometric counts were obtained few minutes, compared 2 d required counts. Under ideal conditions, each bacterial examined exhibited characteristic 'signature' on cytometer, which could be explained its known properties morphology.