NMR Studies on the syn-anti Dynamic Equilibrium in Purine Nucleosides and Nucleotides
作者： Ryszard STOLARSKI , Lech DUDYCZ , David SHUGAR
DOI: 10.1111/J.1432-1033.1980.TB04701.X
关键词:
摘要: The syn in equilibrium anti conformation about the glycosidic bond of purine nucleosides and 5'-nucleotides different solvent systems has been investigated by means 1H NMR spectroscopy. Quantitative values for conformer populations were improved, relative to previous results, a detailed study of, resultant derived correction for, influence sugar exocyclic group on chemical shifts ring protons. This was achieved with aid nucleotides fixed conformations gauche-trans [derivatives 8,5'-(R)-cyclo] trans-gauche 8,5'-(S)-cyclo]. results 13C confirmed those obtained NMR. measured vicinal coupling constants between H-1' C-8 C-4 carbons employed evaluate approximately angles chi anti. A critical examination is made applicability relaxation methods, involving analysis spin-lattice time protons (T1) Overhauser effect, determine base bond; interpretations are provided lack agreement these methods based present study. foregoing resuls also applied an effect enzymatic reactions catalyzed 5'-nucleotidase adenosine deaminase.
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