Polyethylene Glycol Diisocyanate Decreases Platelet Deposition After Balloon Injury of Rabbit Femoral Arteries

摘要: Background: Platelet deposition after angioplasty remains problematic and may contribute to intimal hyperplasia restenosis. We proposed that polyethylene glycol diisocyanate (PEG-DISO), a polymer rapidly forms covalent linkages with amine residues on proteins, could mask thrombogenic vascular wall proteins from platelets, thereby abrogating acute platelet deposition. Methods Results: To test this hypothesis, we isolated the femoral arteries of 10 New Zealand White rabbits injured them 3 passes 2F Fogarty catheter which was inserted through distal arteriotomy. Immediately balloon injury, 111indium-labeled autologous platelets were infused peripherally randomly treated for 1 minute PEG-DISO solution in one artery control phosphate buffered saline vehicle contralateral artery. Following treatment, reflow initiated. The vessels harvested hour radioactivity quantified gamma counter. counts standardized by weight expressed as platelets/mg (mean ± SEM). onto (1.2 0.5) × 106 compared (5.6 4.2) (P < 0.005). Scanning electron micrographs vessel segment confirmed qualitatively less segments than segments. Conclusion: Treatment significantly inhibited injury. These data support hypothesis treatment masks surface adhesive receptors vivo resulting molecular barrier reduces damaged at least hour. formation molecularly thin thus be novel effective abrogate intravascular thrombosis have value