Molecular Cloning and Characterization of a Microsatellite Locus Found in an RAPD Marker of a Spider Mite, Panonychus Citri (Acari: Tetranychidae)
作者： Mh. Osakabe , Norihide Hinomoto , Satoshi Toda , Shinkichi Komazaki , Koichi Goka
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摘要: Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychuscitri. A region consisting a variable number CT tandem repeats (microsatellite) was found fragment amplified the OPB10 primer. The microsatellite differed size by ca. 100 bp among several P. citri populations screened and derived from at least seven alleles. This characteristic mori osmanthi, but lacking ulmi. flanking regions highly conserved these species.
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