Microscopical studies on fungal development and host responses in broad bean and tulip leaves inoculated with five species of Botrytis

摘要: Fungal development and host responses were examined in broad bean tulip leaves inoculated with Botrytis cinerea, B. elliptica, fabae, squamosa tulipae. There was little difference between species their rates of germination under the conditions employed but pathogenic fabae tulipae clearly distinguished from non-pathogens by rapid penetration formation infection hyphae respective hosts, Vicia faba Tulipa gesneriana. At high inoculum concentration produced tulipe grew at linear rates, reaching lengths >;200 μm after one day. By contrast only short hyphae, typically restricted <50 pm achieved during first day inoculation. Many died resistant reactions non-host plants. Reduction greatly reduced growth hosts led to death complete (B. fabae) or some constituent cells tulipae). Formation associated several epidermal around sites penetration. The numbers killed increased rapidly 6 12 h inoculation particularly striking fabae. Penetration caused fewer occasionally formed without cell death, Deposition reaction material (papillae) observed attempted non-pathogenic species. Reaction both plants fluoresced yellow u.v. radiation. Fluorescent deposits gave positive histochemical tests for lignin. Deposits not lignified contained phenolic material. The entirely necrotrophic is discussed reference physiological mechanisms underlying specificity amongst Botrytis.