Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp. I. In vitro embryo rescue and plant development

摘要: Abstract Seedless grape cultivars (Vitis vinifera L.) are widely grown in Europe, America and Asia. Fungal diseases a great threat of them. Several wild Chinese Vitis species showed high resistance to many fungal diseases. Therefore, an investigation was conducted assess the potential incorporate these breeding project for development disease-resistant seedless cultivars. Hybridization using V. as female parents spp. male parents. In-ovulo embryo rescue used develop hybrid plants from females. An efficient protocol is reported here vitro plant cross Emerald Seedless × Beichun. Ovules were excised immature fruits 7 weeks after pollination (WAP) cultured double-phase ER medium supplemented with 6.0% sucrose 0.3% activated charcoal (AC). Following 8–12 culture, embryos removed ovules transferred onto WPM 1.0 μM 6-benzyladenine (6-BA), 2.0% 0.2% AC solidified 0.6% agar. After 8 germinated subsequently grew into whole plantlets. With optimized parameters developed present study, about 34.0%, 91.2% 77.4% formation, germination obtained, respectively. When this applied 11 other combinations, genotype found significantly influence development, different frequencies successfully obtained all crosses.