Pharmacologically active microcarriers delivering BDNF within a hydrogel: Novel strategy for human bone marrow-derived stem cells neural/neuronal differentiation guidance and therapeutic secretome enhancement.
作者： Saikrishna Kandalam , Laurence Sindji , Gaëtan J-R Delcroix , Fabien Violet , Xavier Garric
DOI: 10.1016/J.ACTBIO.2016.11.030
关键词:
摘要: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem and pharmacologically active microcarriers (PAMs) into an non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain cell factor delivery device. It should direct survival and/or neuronal differentiation grafted cells, safely transplant them central nervous system, enhance their tissue repair properties. A model protein was used optimize nanoprecipitation conditions neuroprotective brain-derived neurotrophic (BDNF). BDNF nanoprecipitate encapsulated fibronectin-coated (FN) PAMs vitro release profile evaluated. showed a prolonged, bi-phasic, bioactive BDNF, without burst effect. demonstrated that Si-HPMC hydrogel increased expression neural/neuronal markers MIAMI after 1 week. Moreover, 3D environment (PAMs or hydrogel) secretion (b-NGF, SCF, HGF, LIF, PlGF-1, SDF-1α, VEGF-A & D) chemokines (MIP-1α β, RANTES, IL-8). These results show delivering represent useful novel local tool context not only provides but also bone marrow-derived benefit from by displaying neural commitment improved neuroprotective/reparative secretome. preliminary evidence promising pro-angiogenic, axonal growth-promoting device for system.
archives-ouvertes.fr
elsevier.com
sci-hub.se 参考文章(60)
Albert Kim, James M Conner, Edward Rockenstein, Moses V Chao, Edward H Koo, Daniel Geschwind, Eliezer Masliah, Andrea A Chiba, Mark H Tuszynski, Alan H Nagahara, David A Merrill, Giovanni Coppola, Shingo Tsukada, Brock E Schroeder, Gideon M Shaked, Ling Wang, Armin Blesch, Neuroprotective effects of brain-derived neurotrophic factor in rodent and primate models of Alzheimer's disease Nature Medicine. ,vol. 15, pp. 331- 337 ,(2009) , 10.1038/NM.1912
Emily R. Aurand, Kyle J. Lampe, Kimberly B. Bjugstad, Defining and designing polymers and hydrogels for neural tissue engineering Neuroscience Research. ,vol. 72, pp. 199- 213 ,(2012) , 10.1016/J.NEURES.2011.12.005
Albert Giralt, Olga Carretón, Cristina Lao-Peregrin, Eduardo D Martín, Jordi Alberch, Conditional BDNF release under pathological conditions improves Huntington's disease pathology by delaying neuronal dysfunction Molecular Neurodegeneration. ,vol. 6, pp. 71- 71 ,(2011) , 10.1186/1750-1326-6-71
Yan Jin, Yalei Zhou, Zhiwei Yan, Hongmei Zhang, Wei Lu, Shiyu Liu, Xinhui Huang, Hailang Luo, Expansion and delivery of adipose-derived mesenchymal stem cells on three microcarriers for soft tissue regeneration. Tissue Engineering Part A. ,vol. 17, pp. 2981- 2997 ,(2011) , 10.1089/TEN.TEA.2010.0707
Nicolas Daviaud, Elisa Garbayo, Laurence Sindji, Alberto Martínez-Serrano, Paul C Schiller, Claudia N Montero-Menei, None, Survival, Differentiation, and Neuroprotective Mechanisms of Human Stem Cells Complexed With Neurotrophin-3-Releasing Pharmacologically Active Microcarriers in an Ex Vivo Model of Parkinson’s Disease Stem Cells Translational Medicine. ,vol. 4, pp. 670- 684 ,(2015) , 10.5966/SCTM.2014-0139
Samia Laïb, Borhane H Fellah, Ahmed Fatimi, Sophie Quillard, Claire Vinatier, Olivier Gauthier, Pascal Janvier, Marc Petit, Bruno Bujoli, Sylvain Bohic, Pierre Weiss, The in vivo degradation of a ruthenium labelled polysaccharide-based hydrogel for bone tissue engineering. Biomaterials. ,vol. 30, pp. 1568- 1577 ,(2009) , 10.1016/J.BIOMATERIALS.2008.11.031
Wei-Hsin Chen, Sin-Jhong Cheng, Jason T. C. Tzen, Chao-Min Cheng, Yi-Wen Lin, Probing Relevant Molecules in Modulating the Neurite Outgrowth of Hippocampal Neurons on Substrates of Different Stiffness PLoS ONE. ,vol. 8, pp. e83394- ,(2013) , 10.1371/JOURNAL.PONE.0083394
Marie Morille, Tran Van-Thanh, Xavier Garric, Jérôme Cayon, Jean Coudane, Danièle Noël, Marie-Claire Venier-Julienne, Claudia N. Montero-Menei, New PLGA–P188–PLGA matrix enhances TGF-β3 release from pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal stem cells Journal of Controlled Release. ,vol. 170, pp. 99- 110 ,(2013) , 10.1016/J.JCONREL.2013.04.017
Akhilesh Banerjee, Manish Arha, Soumitra Choudhary, Randolph S. Ashton, Surita R. Bhatia, David V. Schaffer, Ravi S. Kane, The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells Biomaterials. ,vol. 30, pp. 4695- 4699 ,(2009) , 10.1016/J.BIOMATERIALS.2009.05.050
Carmela Giampà, Elena Montagna, Clemente Dato, Mariarosa A. B. Melone, Giorgio Bernardi, Francesca Romana Fusco, Systemic Delivery of Recombinant Brain Derived Neurotrophic Factor (BDNF) in the R6/2 Mouse Model of Huntington’s Disease PLoS ONE. ,vol. 8, pp. e64037- ,(2013) , 10.1371/JOURNAL.PONE.0064037