Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes.

摘要: Abstract Paclitaxel (PTX), a widely used anticancer agent, is found in the inner bark of several Taxus species, although at such low levels that its extraction ecologically unsustainable. Biotechnological platforms based on sp. cell cultures offer an eco-friendlier approach to PTX production, with yields can be improved by elicitation. However, also limited excretion target compounds from producer cells medium hampers their and purification. In this context, we studied effect treating T. media elicitor coronatine (COR) calix[8]arenes (CAL), nanoparticles host lipophilic within macrocyclic scaffold. The highest taxane production (103.5 mg.L-1), achieved after treatment COR (1 μM) CAL (10 was 15-fold greater than control, represented 82% total taxanes analyzed. Expression flux-limiting biosynthetic genes, BAPT DBTNBT, increased addition COR, confirming action, but not CAL. significantly enhanced excretion, especially when were COR; 98% culture COR+CAL treatment. By forming complexes PTX, facilitated medium, protecting toxicity, intra-and extra-cellular degradation may have been avoided. therefore bio-sustainable economically viable system improve yield important compound.