Selective Aptamers for Detection of Estradiol and Ethynylestradiol in Natural Waters
作者： Spurti U. Akki , Charles J. Werth , Scott K. Silverman
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摘要: We used in vitro selection to identify new DNA aptamers for two endocrine-disrupting compounds often found in treated and natural waters, 17β-estradiol (E2) and 17α-ethynylestradiol (EE). We used equilibrium filtration to determine aptamer sensitivity/selectivity and dimethyl sulfate (DMS) probing to explore aptamer binding sites. The new E2 aptamers are at least 74-fold more sensitive for E2 than is a previously reported DNA aptamer, with dissociation constants (K d values) of 0.6 μM. Similarly, the EE aptamers …
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