The adult cystic fibrosis airway microbiota is stable over time and infection type, and highly resilient to antibiotic treatment of exacerbations.

摘要: Cystic fibrosis (CF) is characterized by defective mucociliary clearance and chronic airway infection a complex microbiota. Infection, persistent inflammation periodic episodes of acute pulmonary exacerbation contribute to an irreversible decline in CF lung function. While the factors leading exacerbations are poorly understood, antibiotic treatment can temporarily resolve symptoms partially restore Previous studies indicated that may be associated with changes microbial densities acquisition new species. Given complexity microbiota, we applied massively parallel pyrosequencing identify community structure 23 adult patients during exacerbation, after periods stable disease. Over 350,000 sequences were generated, representing nearly 170 distinct taxa. Approximately 60% obtained from recognized pathogens Pseudomonas Burkholderia, which detected largely non-overlapping patient subsets. In contrast, other taxa including Prevotella, Streptococcus, Rothia Veillonella abundant all samples. Although was small decrease species richness, there minimal change overall structure. Furthermore, composition highly similar when clinically stable, suggesting represent intrapulmonary spread rather than composition. Mouthwash samples, subset patients, showed identical distribution as expectorated sputum, indicating aspiration colonization lower airways. Finally, observed strong correlation between low richness poor Taken together, these results indicate microbiome through short-term compositional microbiota do not account for exacerbations.