Morphological changes ofGiardia lambliacysts after treatment with ozone and chlorine

摘要: Morphological changes of Giardia lamblia cysts after treatment with ozone, free chlorine, or combinations oxidants were documented using transmission electron microscopy. Experiments conducted in vitro-derived G. cysts. Nucleic acid staining employing LIVE/DEAD BacLightTM was used for the evaluation cyst viability. Cyst wall damage, plasmalemma breakage, lysis peripheral vacuoles, and nuclear degradation main features injury elicited by ozone chlorine. Cell death pathways found to be oxidant-specific. treated both chlorine showed that preconditioning exposure first oxidant allowed easier penetration increased damage induced second oxidant. Taken together, our results indicate pretreatment low doses one significantly increases susceptibility parasite sequentially applied Key words: o...