Chapter 4 The Neurospora crassa plasma membrane H+ -ATPase

摘要: Publisher Summary This chapter discusses the structure and dynamics of Neurospora ATPase other P-type ATPases, allowing formulation an approximate structural model for these enzymes. It information available on catalytic mechanism H + -ATPase enzymes within framework this model. From considerations, relatively straightforward schemes molecular mechanisms by which ATPases might catalyze ATP hydrolysis-driven ion translocation can be visualized. Progress toward understanding any enzyme usually depends ease with isolated, amounts obtainable, stability final preparation. The gene sequence indicates presence eight cysteine residues in molecule. To ascertain chemical state residues, direct studies established cystine reagents have been carried out. Titrations reagent—dithiobisnitrobenzoate—and reagent—nitrothiosulfobenzoate—indicated six free cysteines one disulfide bridge