A PCR approach to discriminate between integrated and episomal HPV DNA in small clinical specimens

摘要: HPV infection has long been implicated in the development of cervical carcinoma. There is strong evidence for association high-risk types 16 and 18 with intraepithelial neoplasia (CIN) grades 2 3, integration viral DNA these into host genome suggested to play an important role progression invasive However, existing techniques detection integrated clinical specimens are time-consuming require large amounts template DNA, often unavailable small premalignant CIN lesions. In this study, a novel, two stage PCR assay was designed discriminate between episomal DNA. The first determine whether intact genomes were present. This initial analysis entire eight segments successfully applied authentic human cancers. second consisted ANCHOR-PCR-based analysis, developed specifically that tested cloned containing plasmids used mimic both Further optimization validation will be required application specimens. applicable colposcopic biopsies or scrape samples, representative those acquired routine investigation which determination physical state may provide prognostically valuable information.