miR‐370 promotes high glucose‐induced podocyte injuries by inhibiting angiotensin II type 1 receptor‐associated protein
作者： Yuxin Xian , Liping Dong , Yong Jia , Yi Lin , Wenjuan Jiao
DOI: 10.1002/CBIN.11048
关键词:
摘要: miRNAs expression profiles in podocyte injuries have been reported different models vivo and vitro. In the present study, miR-370 was elevated high glucose-stimulated podocyte, post-transcriptional mechanism of investigated glucose-induced injuries. The gene protein levels were assayed by RT-qPCR Western blotting, respectively. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining used to evaluate apoptosis podocyte. targeted genes predicted a bioinformatics algorithm confirmed dual luciferase reporter assay. results demonstrated that over-activation verified glucose-treated podocytes, while repression protected against apoptosis, cell membrane, DNA damage podocytes. We also found AGTRAP as direct target served an opposite effect miR-370, overexpression blocked podocytes dysfunction. conclusions, activating signaling inhibit AGTRAP, representing novel promising therapeutic for treatment DN.
sci-hub.se 参考文章(34)
Lili Zhou, Youhua Liu, Wnt/β-catenin signalling and podocyte dysfunction in proteinuric kidney disease. Nature Reviews Nephrology. ,vol. 11, pp. 535- 545 ,(2015) , 10.1038/NRNEPH.2015.88
Li-Juan Min, Masaki Mogi, Kouichi Tamura, Jun Iwanami, Akiko Sakata, Teppei Fujita, Kana Tsukuda, Fei Jing, Masaru Iwai, Masatsugu Horiuchi, Angiotensin II type 1 receptor-associated protein prevents vascular smooth muscle cell senescence via inactivation of calcineurin/nuclear factor of activated T cells pathway Journal of Molecular and Cellular Cardiology. ,vol. 47, pp. 798- 809 ,(2009) , 10.1016/J.YJMCC.2009.09.006
Tsukasa Nakamura, Eiichi Sato, Mayuko Amaha, Yasuhiro Kawagoe, Sayaka Maeda, Sho-ichi Yamagishi, Addition of aliskiren to angiotensin II receptor blockers ameliorates renal tubular injury and reduces intima media thickness of carotid artery in patients with diabetic nephropathy. International Journal of Cardiology. ,vol. 155, pp. 294- 296 ,(2012) , 10.1016/J.IJCARD.2011.11.080
Hans Clevers, Roel Nusse, Wnt/β-catenin signaling and disease. Cell. ,vol. 149, pp. 1192- 1205 ,(2012) , 10.1016/J.CELL.2012.05.012
Partab Rai, Tejinder Singh, Rivka Lederman, Amrita Chawla, Dileep Kumar, Kang Cheng, Gautam Valecha, Peter W. Mathieson, Moin A. Saleem, Ashwani Malhotra, Pravin C. Singhal, Hyperglycemia Enhances Kidney Cell Injury in HIVAN through down regulation of vitamin D receptors Cellular Signalling. ,vol. 27, pp. 460- 469 ,(2015) , 10.1016/J.CELLSIG.2014.12.011
Benoit Denhez, Farah Lizotte, Marie-Odile Guimond, Nina Jones, Tomoko Takano, Pedro Geraldes, Increased SHP-1 Protein Expression by High Glucose Levels Reduces Nephrin Phosphorylation in Podocytes Journal of Biological Chemistry. ,vol. 290, pp. 350- 358 ,(2015) , 10.1074/JBC.M114.612721
S-S Lo, P-S Hung, J-H Chen, H-F Tu, W-L Fang, C-Y Chen, W-T Chen, N-R Gong, C-W Wu, Overexpression of miR-370 and downregulation of its novel target TGFβ-RII contribute to the progression of gastric carcinoma. Oncogene. ,vol. 31, pp. 226- 237 ,(2012) , 10.1038/ONC.2011.226
Claudiane Guay, Romano Regazzi, Circulating microRNAs as novel biomarkers for diabetes mellitus Nature Reviews Endocrinology. ,vol. 9, pp. 513- 521 ,(2013) , 10.1038/NRENDO.2013.86
D. L. Jennings, J. S. Kalus, C. I. Coleman, C. Manierski, J. Yee, Combination therapy with an ACE inhibitor and an angiotensin receptor blocker for diabetic nephropathy: a meta-analysis. Diabetic Medicine. ,vol. 24, pp. 486- 493 ,(2007) , 10.1111/J.1464-5491.2007.02097.X
Marco Lopez-Ilasaca, Xiushi Liu, Koichi Tamura, Victor J. Dzau, The Angiotensin II Type I Receptor-associated Protein, ATRAP, Is a Transmembrane Protein and a Modulator of Angiotensin II Signaling Molecular Biology of the Cell. ,vol. 14, pp. 5038- 5050 ,(2003) , 10.1091/MBC.E03-06-0383