Evaluation of a fluid versus a powder pepsin formulation to detect Trichinella spiralis larvae in meat samples by a digestion technique.

摘要: Pepsin powder constitutes a health risk, potentially causing severe allergic reactions to those handling the chemical. A fluid pepsin formulation was produced and tested, first in preliminary study then ring trial encompassing four European National Reference Laboratories (NRLs). The purpose of each ascertain compare action with that for digesting meat liberating encapsulated Trichinella spiralis larvae subsequent counting. quality digestion furthermore evaluated by assessing visibility through amount debris remaining after digestion. For trial, at laboratory 20 blinded replicate 100-g samples pork containing known number T. (0 30) were digested magnetic stirrer method using either standard (10 samples) or samples). With an average recovery rate 70 80%, all NRLs found be equally effective. also no difference between two formulations regard remnants fluid. use may therefore constitute improvement procedure analysts involved.