Novel bioluminescent binding assays for interaction studies of protein/peptide hormones with their receptors

摘要: Protein/peptide hormones are the largest group of endogenous signaling molecules and exert various biological functions by binding to specific cell membrane receptors. To study interactions between these their receptors, quantitative ligand–receptor assays have been widely used for decades. However, conventionally relied on use radioligands, which some major drawbacks can only be in laboratories with a radioactive material license. We recently developed novel bioluminescent several protein/peptide using brightest reporter known date, nanoluciferase (NanoLuc). The NanoLuc either chemically conjugated an appropriate position, or genetically fused at one terminus, hormones. Compared conventional ligands higher sensitivity, better safety, longer shelf lives, thus, represent class non-radioactive tracers receptor assays. In present review, we provide general considerations examples setting up Such techniques applied other future facilitate interaction studies